Stress in dogs can be assessed using physiological measures and behavioural observations. Behavioural indicators involve vocalisation, panting, lowered posture, paw lifting, body shaking, and stereotypic behaviours (Beerda et al. 2000).
Environmental enrichment has been increasingly studied to enhance the welfare of kenneled or confined dogs (Wells 2009). Auditory enrichment reduces stress, supports species-specific behaviours, and minimises abnormal behaviours. Wells et al. (2002) reported that dogs exposed to classical music had more resting, less standing, and reduced vocalisation than other auditory stimuli, suggesting a calming effect. Bowman et al. (2015) confirmed that classical music can reduce behavioural and physiological stress responses in shelter dogs. Their findings also suggest sex-based differences, with males being more responsive to auditory enrichment. These results are consistent with the findings of this study.
Several factors should be considered when implementing auditory enrichment. Music must not contain extreme frequencies and should remain below 60 dB (Patterson-Kane and Farnworth, 2006). In noisier environments, such as grooming salons, the volume may need to be increased to mask aversive sounds. Dogs show elevated blood glucose levels in response to noise above 80 dB (Treptow, 1966), indicating a stress reaction (Kuo et al., 2015).
Not all auditory stimuli have calming effects. Harsh or abrupt sounds may increase arousal or fear (Köster et al., 2019). Individual variability plays a significant role in auditory response. Hearing ability, which may decline with age or differ by breed, also influences effectiveness (Fefer et al., 2022).
This study selected Beethoven’s Moonlight Sonata and Chopin’s Nocturne in E-flat major due to their slow tempo, soft dynamics, and absence of vocals or abrupt changes. These piano pieces, with frequencies between 104 Hz and 1.3 kHz, fall within the auditory range of dogs and humans. Although the study examined differences in dogs' responses to minor and major keys, no statistically significant differences were found. Both pieces, with tempos around 60–80 BPM, may entrain biological rhythms and contribute to stress reduction.