RUNX1-mediated LINC01094 promotes gastric cancer through dual targeting of CDKN1A by directly binding RBMS2 and HDAC1

doi:10.21203/rs.3.rs-5112702/v1

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Research Article

RUNX1-mediated LINC01094 promotes gastric cancer through dual targeting of CDKN1A by directly binding RBMS2 and HDAC1

https://doi.org/10.21203/rs.3.rs-5112702/v1

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published 25 Dec, 2024

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Background

Accumulating studies have focused on long noncoding RNAs (lncRNAs) because of their regulatory effects on multiple cancers. However, the biological functions and molecular mechanisms of lncRNAs in gastric cancer (GC) remain to be elucidated in depth.

Methods

Long intergenic nonprotein coding RNA 1094 (LINC01094), a differentially expressed lncRNA between GC tissues and adjacent normal tissues, was identified. Moreover, gain- and loss-of-function experiments in vitro and in vivo were carried out. To understand the mechanisms underlying the regulatory effects of LINC01094, we performed RNA pull-down assays, RNA immunoprecipitation assays, chromatin immunoprecipitation assays, luciferase reporter assays, etc.

Results

LINC01094 was markedly upregulated in GC tissues and cell lines, and LINC01094 upregulation was positively correlated with GC malignant behaviours in vitro and in vivo. Mechanistically, LINC01094 downregulated the expression of CDKN1A by interacting with RNA binding motif single stranded interacting protein 2 (RBMS2) and histone deacetylase 1 (HDAC1). Additionally, LINC01094 was confirmed to sponge miR-128-3p and participate in the LINC01094-miR-128-3p-RUNX family transcription factor 1 (RUNX1) feedback loop. Finally, Ro 5-3335, a validated RUNX1 inhibitor, was explored for anticancer drug development in GC.

Conclusions

The LINC01094-miR-128-3p-RUNX1 feedback loop downregulates CDKN1A and promotes GC cooperatively with RBMS2 and HDAC1. Moreover, Ro 5-3335 may have promising therapeutic applications in GC. Hence, our study found an oncogenic lncRNA, LINC01094, which could be a promising target for cancer treatment and diagnosis.

gastric cancer

LINC01094

CDKN1A

RUNX1

RNA-binding protein

As one of the deadliest malignant gastrointestinal tumors, gastric cancer (GC) resulted in at least one million new cases and approximately 0.76 million deaths in 2020 worldwide [1]. Despite developments and improvements in GC diagnosis and treatment resulting from many related studies, surgical resection remains the only potentially curative option for GC patients [2]. Thus, it is essential to seek pivotal molecular targets and potential regulatory networks of GC.

Long noncoding RNAs (lncRNAs) are RNA molecules containing more than 200 nucleotides (nt) and are reported to have limited protein-coding potential [3]. Accumulating evidence indicates that lncRNAs can interact with multiple biological molecules and play roles as parts of complex regulatory networks in various diseases, including cancers [4–6]. Although the abilities of lncRNAs to function as competitive endogenous RNAs (ceRNAs) and sponge microRNAs have been extensively studied [7, 8], it is worth further exploring whether lncRNAs can bind proteins and play roles in diverse key biological processes. Some lncRNAs can recruit transcription factors and regulate chromosome status [9, 10], whereas some lncRNAs can affect posttranslational and posttranscriptional modifications [11, 12].

LINC01094 is a cancer-promoting molecule correlated with many classical signaling pathways, such as the Wnt/β-catenin pathway and PI3K/AKT pathway [13–15]. Many bioinformatic studies have predicted the potential of LINC01094 to affect angiogenesis, autophagy, epithelial-to-mesenchymal transition (EMT), and macrophage infiltration in GC [16–18]. Our study was designed to comprehensively assess the expression level and biological function of LINC01094. Then, we explored the underlying mechanisms based on its unique subcellular localization pattern in GC cell lines. Additionally, the regulator of LINC01094 expression was predicted, and a drug targeting the potential molecule was preliminarily validated.

Tissue samples

Eighty GC tissue samples and the corresponding adjacent normal tissues were obtained from the First Affiliated Hospital of Nanjing Medical University between April 2015 and April 2021. All patients were definitively diagnosed by at least two pathologists to have no history of preoperative chemoradiotherapy or other malignancies. The clinicopathological characteristics of the patients were obtained from medical records, and the survival status was ascertained through annual telephone interviews. After informed consent was obtained from each participant, our study was evaluated and approved by the Ethics Committee of the First Affiliated Hospital of Nanjing Medical University.

Cell culture

The cell lines used in this study, including HEK-293T, the normal human glandular epithelial cell line GES-1, and several common human GC cell lines, were provided by the Cell Center of Shanghai Institutes for Biological Sciences. HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM H-21 4.5 g/litre glucose) (Wisent, Canada), and AGS cells were cultured in Nutrient Mixture F-12K medium (Wisent, Canada). The other aforementioned cell lines were cultured in RPMI 1640 medium. Foetal bovine serum (FBS; 10%, Wisent, Canada) was added to the aforementioned media along with 1% penicillin-streptomycin. All cell lines were incubated in a humidified atmosphere of 5% CO2 at 37°C.

Plasmid construction, siRNA transfection, and lentiviral infection

The human LINC01094 overexpression vector was constructed by inserting the corresponding cDNA sequence into the pcDNA3.1 vector (Obio, China), with the empty vector used as a control. Small interfering RNAs (siRNAs) against LINC01094 (GenePharma, China) were synthesized, with a scrambled siRNA used as a negative control. Lipofectamine 3000 (Invitrogen, USA) was used for transfection. The sequences of the LINC01094 cDNA and the siRNAs targeting LINC01094 and cyclin-dependent kinase inhibitor 1A (CDKN1A) were inserted into a lentiviral vector (GenePharma, China). Specific plasmids expressing and siRNAs targeting RNA binding motif single stranded interacting protein 2 (RBMS2), histone deacetylase 1 (HDAC1), CDKN1A, and RUNX family transcription factor 1 (RUNX1) were constructed as described above. Expression at different levels was evaluated by quantitative real-time reverse transcription polymerase chain reaction (qRT‒PCR) to validate the gene knockdown and overexpression efficiencies in the supplementary material (Fig. S1A-B and Fig. S3A-D). The related sequences were used in previous studies and are listed in the supplementary material [19–25].

RNA extraction and qRT-PCR

Total RNA was extracted from cells and tissues by TRIzol reagent (Invitrogen, USA). The New Poly (A) Tailing Kit (Thermo Fisher Scientific, USA) and the Prime Script RT Reagent Kit (Takara, Japan) were used for the reverse transcription of miRNA and lncRNA/mRNA, respectively. Amplification was performed with SYBR Green Master Mix (Roche, China). LncRNA and mRNA expression were normalized to GAPDH expression. Related procedures were performed three times, and the 2^−ΔΔCT method was applied to evaluate expression levels. The primers used in this study are described in the supplementary material.

Nuclear-cytoplasmic fractionation

The PARIS™ kit (Thermo Fisher Scientific, USA) was used for isolation of the nuclear and cytoplasmic fractions according to the manufacturer’s steps. After lysing and centrifuging the cells, we obtained the supernatant as the cytoplasmic fraction. Subsequently, we washed the pelleted nuclei with a Cell Fraction Buffer and collected the nuclear fraction.

mRNA stability and protein stability analyzes

For mRNA stability analysis, we treated MKN45 cells with 5 µg/mL actinomycin D (ActD; Sigma-Aldrich, USA) and measured target gene expression at 2-hour intervals (to 12 h). Cycloheximide (CHX; 50 µg/mL; Sigma-Aldrich, USA) was used for protein stability analysis. The remaining procedures were similar to those described above.

Fluorescence in situ hybridization (FISH) assay

Fluorescently labelled LINC01094 and miR-128-3p probes were constructed, and 4’,6-diamidino-2-phenylindole (DAPI) was applied to stain nuclei. The probe signals in GC cells and tissues were detected with a FISH kit (RiboBio, China). Images were obtained with confocal microscopy (Carl Zeiss, Germany).

RNA pull-down assay

According to the instructions, we incubated a biotin-labeled LINC01094 probe (RiboBio, China) with streptavidin magnetic beads. After probe-coated beads were generated, we obtained MKN45 cell lysates, incubated them with the aforementioned beads overnight, and then washed the beads. Then, we performed silver staining with a silver staining kit (Thermo Fisher Scientific, USA). Furthermore, we performed mass spectrometry and western blotting to analyze the precipitated proteins.

RNA immunoprecipitation (RIP) assay

Following the protocol of the Magna RIP RNA Binding Protein Immunoprecipitation Kit (Millipore, USA), we incubated magnetic beads with specific antibodies at 25°C and mixed the GC cell lysates with the antibody-bead complexes at 4°C. Then, qRT-PCR was applied to quantify the eluted bound RNAs.

Chromatin immunoprecipitation (ChIP) assay

We purchased the Magna ChIP™ A/G Kit (Millipore, USA) for the ChIP assay. After fixing the cells with 1% formaldehyde and quenching the crosslinking reaction, we generated 200-1000-bp fragments of the crosslinked chromatin by sonication. The magnetic beads were incubated with related antibodies and cell lysates. Then, qRT-PCR was applied to analyze the eluted immunoprecipitated complexes.

Triplex capture assay

We incubated MKN45 cell nuclei with 2 µg biotinylated triplex-forming oligonucleotides (TFOs) and subjected them to UV (365 nm) irradiation and sonication. After centrifugation of the nuclei, the supernatant was separated and incubated with streptavidin-magnetic beads (Thermo Fisher Scientific, USA) at 4°C. Following DNA extraction and purification and qRT-PCR analysis, we washed the beads 5 times and then resuspended the eluted complexes. The primers used are described in the supplementary material.

Luciferase reporter assay

The corresponding fragments were synthesized and subcloned into the luciferase reporter plasmid (Realgene, China). Various gene overexpression plasmids and siRNAs were cotransfected with the luciferase reporter plasmid. Then, we collected the cells and applied the Dual-Luciferase Assay Kit (Promega, USA). Firefly and Renilla luciferase activities were measured and are presented as relative luciferase activity values (firefly luciferase/Renilla luciferase).

Western blot analysis

The western blot protocol was consistent with that used in our previous study, and the antibodies used are described in the supplementary material [26].

Immunohistochemical (IHC) and hematoxylin & eosin (HE) staining

For IHC staining, paraffin-embedded tissues were dewaxed, rehydrated, and subjected to antigen retrieval. Specific antibodies were incubated with the sections, and expression levels were evaluated with diaminobenzidine (DAB) the next day.

Paraffin-embedded mouse lung tissues were sliced into 5-µm sections and stained with HE for pathologic analysis.

Functional assays

Cell proliferation, migration, and invasion assays

To observe the effects on cell proliferation, migration, and invasion, Cell Counting Kit-8 (CCK-8), colony formation, wound healing, and transwell assays were performed as described in our previous study [26].

Flow cytometric analysis

The Annexin V-FITC/PI Apoptosis Detection Kit (Multisciences, China) and the Cell Cycle Analysis Kit (Multisciences, China) were applied to evaluate the apoptosis rate and the cell cycle, respectively, according to the corresponding protocols. The apoptosis rate was calculated as the sum of early apoptotic and late apoptotic cells. Cell cycle data were divided into G1, S as well as G2/M phases and analyzed by FlowJo (v10.8.1).

Animal experiments

Cell suspensions (0.1 ml, 1 × 10⁶ stable cells) were subcutaneously injected into four-week-old male nude mice (BALB/c) to establish the xenograft model. We measured the tumor volume every 2 days and weighed the xenografts after sacrificing the mice 2 weeks after cell injection. For the lung metastasis model, we injected cells into the caudal vein of nude mice, and metastatic tumors were detected by HE staining. The care of animals involved in this study was in accordance with the National Research Council's Guide for the Care and Use of Laboratory Animals.

GC organoid model

Human GC organoids model were constructed as described previously [27]. We transfected the LINC01094 shRNA and its negative control into the organoids to facilitate our investigation of the role of LINC01094 in GC progression. The growth of GC organoid was observed daily by microscope.

Bioinformatic and statistical analyzes

All DNA, RNA, and protein sequences used in this study can be searched in the National Center for Biotechnology Information (NCBI) database. Other public databases and the corresponding websites are listed in the supplementary material. SPSS (version 26.0) and GraphPad Prism (version 8.01) were used for statistical analysis. Student's t test, the Wilcoxon test, and the χ2 test were applied to analyze parametric variables, and the Kaplan‒Meier method was employed to analyze overall survival (OS). Quantitative data are presented as the means ± SDs. Each experiment was repeated independently in triplicate, and p < 0.05 was considered to indicate statistical significance.

Identification and characteristics of LINC01094 in GC

Analysis of the TCGA STAD dataset and a GEO dataset (GSE79973) revealed 19 lncRNAs upregulated in GC (Fig. 1A-C). Among the six survival-associated lncRNAs in GC predicted by the Kaplan‒Meier Plotter website, LINC01094 was identified as overexpressed in GC after qRT‒PCR validation (n = 16) (Fig. 1D-E). This pattern was consistent with that in paired tumor and adjacent tissues (n = 32) in the TCGA STAD dataset (Fig. 1F). To explore the relationships between the LINC01094 expression level and clinicopathological characteristics, qRT‒PCR was performed in a larger sample (n = 80) (Fig. 1G). Statistical analysis showed that a high LINC01094 expression level was positively correlated with invasion depth, distant metastasis, advanced TNM stage, and poor prognosis (Fig. 1H-I). Moreover, higher LINC01094 expression may predict a higher T stage, based on analysis of the TCGA STAD dataset (Fig. 1J), prompting us to hypothesize the effects of LINC01094 on GC cell malignant behaviors. In subsequent functional assays, we measured the LINC01094 expression level in GC cell lines and found that LINC01094 expression was higher in GC cell lines (Fig. 1K). Based on these results, we chose two GC cell lines with intermediate LINC01094 expression levels (MKN45 and AGS) to explore the subcellular localization of LINC01094. In contrast to the prediction of lncATLAS (Fig. 1L), LINC01094 was localized in both the cytoplasm and nucleus of GC cells, as determined by nuclear-cytoplasmic isolation and FISH assays (Fig. 1M-N).

LINC01094 promotes malignant behaviors of GC cells in vitro and in vivo

To evaluate the biological effects of LINC01094 on GC cells, we transfected LINC01094 shRNAs and its negative control into GC cells following by subsequent transcriptome sequencing (Fig. 2A). After molecular pathway enrichment analysis for differentially expressed genes, we noticed that there exists the most significant association between LINC01094 and the process of cell cycle (Fig. 2B). CCK-8 and colony formation assays were performed to evaluate GC cell proliferation, while wound healing and transwell assays were used to assess the migratory and invasive capacities of GC cells. Knockdown of LINC01094 suppressed and overexpression of LINC01094 promoted GC cell proliferation, migration, and invasion (Fig. 2C-G and Fig. S1C-E). Additionally, flow cytometric analysis indicated that higher LINC01094 expression was associated with a lower apoptosis rate and attenuated G1/S arrest (Fig. 2H-I and Fig. S1F-G).

To assess the influence of LINC01094 on GC in vivo, MKN45 cells stably transduced with LV-si-LINC01094 (LINC01094-KD) or the corresponding negative control construct (KD NC) were subcutaneously injected into BALB/c nude mice. Significantly lower volume and weight, as well as a decreased Ki67 level, were observed in LINC01094-KD group tumors compared with KD NC group tumors (Fig. 3A-D and Fig. S1H). In the lung metastasis model, fewer metastatic nodules were detected in the LINC01094-KD group, and this finding was validated by HE staining (Fig. 3E-F). At last, we constructed human GC organoid models to valadate the biological functions of LINC01094. We observed that LINC01094 knockdown remarkbly suppressed the growth of the organoid models (Fig. 3G).

LINC01094 reduces CDKN1A mRNA stability by inhibiting the binding of RBMS2 to the CDKN1A 3’-untranslated region (UTR)

For further analysis, RNA-binding proteins were speculated to be associated with the effects of LINC01094, and an RNA pull-down assay was performed with MKN45 cells to identify the LINC01094-interacting proteins. As the silver staining assay showed, two bands at 44 kDa and 55 kDa exhibiting significant enrichment were observed (Fig. 4A) and were identified to correspond to RBMS2 and HDAC1 by mass spectrometry and western blotting (Fig. 4B-C and Fig. 6A). Then, we performed RIP and obtained the same result (Fig. 4D and Fig. 5B). Regarding the subcellular localization of LINC01094, we assumed that LINC01094 interacted with RBMS2 and HDAC1 in the cytoplasm and nucleus, respectively. The three potential regions where LINC01094 may bind to RBMS2 (111–852 nt, 1412–2240 nt, and 4389–4559 nt) were predicted by the catRAPID database (Fig. 4E). Following RNA pull-down and western blotting, LINC01094 plasmids with individual truncation of the aforementioned regions were constructed and transfected into MKN45 and AGS cells. LINC01094 was confirmed to interact mainly with RBMS2 via its 1412–2240 nt region (Fig. 4F). To identify the specific structural domain of RBMS2 responsible for its binding to LINC01094, RBMS2 mutants with truncated individual structural domains were constructed. The subsequent RIP and RNA pull-down assays revealed that RNA recognition motif 1 (RRM1) of RBMS2 contributed greatly to the interaction of RBMS2 with LINC01094 (Fig. 4G). RBMS2 was reported to directly bind to AU-rich elements (AREs) in the 3’-UTR to improve the stability of CDKN1A mRNA [10, 21, 25]. The production of CDKN1A, namely p21, is considered as an important part of the p53 pathway and can be involved in tumor development by regulating processes such as the cell cycle, which may be the downstream molecule of LINC01094 [28, 29].

For neither the mRNA nor the protein expression of RBMS2 was affected (Fig. 4H-I), we speculated that LINC01094 plays a role by competing with CDKN1A for binding to RBMS2. After preliminary molecular correlation analysis between RBMS2 and CDKN1A in the TCGA STAD dataset (Fig. 5A), RIP assays, qRT‒PCR, and western blotting were performed and showed that RBMS2 can also bind to CDKN1A and increase CDKN1A expression in GC (Fig. 5B-C). Then, differently treated cells were treated with an inhibitor of translation (CHX) or a transcription (ActD). LINC01094 may modulate CDKN1A expression by affecting CDKN1A mRNA stability instead of CDKN1A protein stability, consistent with the effect of RBMS2 (Fig. 5D-E). Further analysis showed that the CDKN1A mRNA half-life was positively correlated with RBMS2 expression (Fig. 5F) and negatively correlated with LINC01094 expression (Fig. 5G). RBMS2 knockdown rescued the effect of LINC01094 downregulation on decreasing the CDKN1A mRNA half-life (Fig. 5H). We constructed reporter vectors containing the p21 3'-UTR and p21 3'-UTR with mutant AREs for luciferase reporter assays and obtained similar results (Fig. 5I-J). For the competition assay, biotin-labeled CDKN1A mRNA was incubated with purified RBMS2 protein containing different amounts of purified LINC01094. LINC01094 was found to promote CDKN1A decay in a dose-dependent manner by competitively binding to RBMS2 (Fig. 5K).

Despite the numerous studies in other tumors [10, 21, 25], the function of RBMS2 in GC has not been reported. Significant differences in RBMS2 expression between tumor tissues and adjacent normal tissues were found by qRT-PCR and western blotting (Fig. S2A-B). In contrast to the findings regarding LINC01094, RBMS2 was found to have obvious tumor-suppressive effects in subsequent functional experiments in vitro (Fig. S2C-I and Fig. S3E-J).

LINC01094 decreases CDKN1A transcription by recruiting HDAC1 to the CDKN1A promoter

HDAC1, a histone deacetylase, is correlated with the dysregulation of tumor suppressor genes in several malignant tumors [30, 31], and we identified it as a protein that binds to LINC01094 (see above). Considering previous studies on RNA‒DNA triplexes [32–34] and the correlations between HDAC1 and CDKN1A [35, 36], we hypothesized that LINC01094 may play a key bridging role in recruiting HDAC1 to the CDKN1A promoter. To validate this hypothesis, a promoter luciferase assay was performed and indicated that LINC01094 could suppress the transcriptional activity of the CDKN1A promoter (Fig. 6C). A report indicated that H3 lysine-9 (H3K9) is the key site in the interaction between HDAC1 and CDKN1A in epidermal progenitor cells [37]. We examined the expression level of H3K9Ac after different treatments (Fig. 6D). Further ChIP assays showed a positive correlation between the LINC01094 expression level and the HDAC1 enrichment level. An inverse correlation was found between LINC01094 expression and H3K9 acetylation (H3K9Ac), the substrate of HDAC1 (Fig. 6E-F). For an in-depth understanding of the RNA‒DNA triplex, we applied the LongTarget website to predict the binding site between LINC01094 and the CDKN1A promoter. The triple target site (TrT) in the CDKN1A promoter was visualized and validated by a triplex capture assay (Fig. 6G). Additionally, the luciferase reporter containing the mutant CDKN1A promoter could not respond to changes in LINC01094 expression when the TrT was not present (Fig. 6H).

LINC01094 exerts biological effects by downregulating CDKN1A

The above results demonstrated that LINC01094 could regulate CDKN1A expression in an intricate manner. The key downstream molecule of LINC01094, CDKN1A is an important component of the p53 signaling pathway and has potent regulatory effects on the cell cycle, apoptosis, and other processes [38, 39]. Western blotting revealed that LINC01094 knockdown or overexpression modulated the expression of CDKN1A and its downstream molecules and that RBMS2 or HDAC1 expression may affect the aforementioned effect of LINC01094 (Fig. 6I). Then, we performed rescue experiments and observed that the attenuation of malignant behaviors after downregulating LINC01094 was partially reversed by CDKN1A knockdown, while the enhanced malignant phenotypes resulting from LINC01094 overexpression were attenuated by CDKN1A upregulation (Fig. 7A-I and Fig. S3L-S). In brief, this series of experiments indicated that the cancer-promoting effects of LINC01094 in GC depend greatly on the LINC01094-RBMS2/HDAC1-CDKN1A axis.

LINC01094 upregulates RUNX1 by sponging miR-128-3p

It has been widely shown that lncRNAs can regulate downstream genes by functioning as miRNA sponges [40, 41]. We applied LncSNP2 and AnnoLnc2 to identify the target of LINC0194, and miR-128-3p was found in both of these online databases (Fig. 8A). After qRT‒PCR validation of the biotin-labelled circSTX6 probe efficiency, miR-128-3p was demonstrated to be significantly enriched in the sponged complexes in MKN45 and AGS cells (Fig. 8B-C). To exclude the possibility of false positives resulting from indirect binding, a biotin-labelled miR-128-3p probe was used in the RNA pull-down assay, and LINC01094 showed marked enrichment with this probe compared with the control probe (Fig. 8D). For miRNAs could bind to Argonaute-2 (AGO2) as RNA-induced silencing complex (RISC) components [42, 43], an RIP assay was performed and both LINC01094 and miR-128-3p were pulled down by the anti-AGO2 antibody (Fig. 8E). Additionally, the potential binding region was visualized, and a dual-luciferase reporter assay was conducted to further characterize the interaction. The miR-128-3p mimics attenuated the luciferase activity of wild-type LINC01094 but not that of mutant-type LINC01094 (Fig. 8F-G). Moreover, the FISH results showed that circNEIL3 was primarily and highly expressed in the GC tissues, while miR-128-3p was mostly located in the adjacent normal tissue. The relationship of co-localization existed between LINC01094 and miR-128-3p (Fig. 8H).

We next sought to identify the target gene of LINC01094 and miR-128-3p. We intersected the predictions of multiple databases to predict the potential downstream gene (Fig. 8I). Through this predictive screening approach, three genes significantly associated with GC prognosis and LINC01094 expression were chosen as the most likely candidates (Fig. 8J). The qRT‒PCR and western blot results showed a negative correlation between miR-128-3p and RUNX1 expression (Fig. 8K). In the luciferase assay, the miR-128-3p mimics decreased luciferase activity in cells transfected with the WT 3’-UTR rather than the MT 3’-UTR of RUNX1 (Fig. 8L). Next, we investigated the association of LINC01094 with RUNX1 and examined whether the effect of LINC01094 on RUNX1 could be reversed by miR-128-3p (Fig. 8M-N).

LINC01094 is upregulated by RUNX1

Interestingly, RUNX1 was identified as the most likely transcription factor targeting LINC01094 by analysis of the JASPAR database (Fig. 9A). RUNX1 regulates oncogenes and has significant impacts on haematologic malignancies [44, 45]. Considering the investigation of the relationship between LINC01094 and RUNX1 (Fig. 9B), a hypothesis regarding a feedback loop was immediately developed. We applied qRT‒PCR to preliminarily confirm this hypothesis (Fig. 9C). The results of subsequent ChIP and luciferase assays confirmed this hypothesis (Fig. 9D and Fig. 9F), indicating that RUNX1 may play a critical role in regulating CDKN1A. Based on the JASPAR database prediction, the five sites located in the LINC01094 promoter region had a relatively high probability of binding directly to RUNX1 (Fig. 9A). Different LINC01094 promoter fragments were amplified in the ChIP assay, with site 2 and site 4 exhibiting higher affinities for RUNX1 than the other sites. Subsequently, a luciferase assay was performed to further determine the binding site. Consistent with previous observations, site 2 and site 4 were required simultaneously during binding (Fig. 9E and Fig. 9G). Moreover, the effect of LINC01094 on CDKN1A expression was influenced by altering RUNX1 expression (Fig. 7H-I). A series of rescue experiments resulted in similar conclusions regarding the function of LINC01094 (Fig. 7J-S and Fig. S4).

As technological developments and understanding have advanced, diverse mechanisms of noncoding RNAs have recently been experimentally verified [46]. Multiple studies have indicated that individual noncoding RNAs can regulate tumor generation and progression in complex and sophisticated manners rather than via a single mechanism [47, 48].

To select the target gene, we first performed differential expression and prognostic analysis. After validating the function of LINC01094 in GC, we performed a deep exploration of the underlying mechanisms. Then, the key molecules and the downstream pathway were identified. Finally, the inhibitor of RUNX1, Ro 5-3335, was used to disrupt the miR-128-3p-RUNX1-LINC01094-RBMS2/HDAC1-CDKN1A axis.

Competitive binding, a key action mechanism of LINC01094, occurs not only between noncoding RNAs and miRNAs but also between noncoding RNAs and proteins. Previous studies have indicated that competitive relationships depend on posttranscriptional modifications, posttranslational modifications, and protein trafficking [49, 50]. RBMS2 was noted to stabilize mRNAs in breast cancer and lung cancer [10, 21], whereas no corresponding research has been conducted in GC. We identified RBMS2 as the downstream molecule of LINC01094 and explored the function of RBMS2 in GC for the first time. Another notable mechanism identified in our study was the formation of an RNA‒DNA triplex. Many triplex-forming motifs exist in gene regulatory regions, especially the promoter region [51]. Accumulated evidence indicates that noncoding RNAs regulate target genes by participating in transcription factor recruitment and RNA‒DNA triplex formation [52, 53]. HDAC1, a member of the HDAC protein family, regulates histone modifications together with other key factors.

The axis that we proposed, a part of the complex molecular networks existing in vivo, merely provided a perspective on the exploration of LINC01094. RUNX1 was reported to inhibit CDKN1A expression through multiple pathways [54, 55], and CDK1, the known downstream gene of CDKN1A, can increase the potency of RUNX1 transactivation by phosphorylating RUNX1 at S48 or S424 [56]. These observations may suggest the existence of a regulatory feedback loop in our proposed axis.

Despite the merits of this study in providing groundbreaking insights and revealing clinical significance, its limitations should be considered. First, we chose our target gene and its downstream molecules by utilizing public databases and published studies. Analyses of corresponding sequencing data may provide more persuasive results. Second, our study involved many regulatory relationships for more comprehensive research. Sufficient rescue experiments were not performed to validate each regulatory relationship. Subsequent correlation analysis of clinical parameters and survival outcomes revealed a strong association between the expression of LINC01094 and HER2 (Fig. 1I), which we did not investigate. This correlation may offer inspiration for further studies. Finally, the effects of Ro 5-3335 on GC seemed to be preliminarily validated. However, much work is required before possible clinical translation.

In summary, we tentatively conclude that LINC01094, regulated by the LINC01094-miR-128-3p-RUNX1 positive feedback loop, downregulates CDKN1A by interacting with RBMS2 and HDAC1 in the cytoplasm and nuclear, respectively, which in turn promotes malignant behaviors and poor prognosis in GC. These results may facilitate future exploration of a potential biomarker and pave the way for the discovery of a therapeutic target for GC.

lncRNAs long non-coding RNAs

GC gastric cancer

LINC01094 long intergenic non-protein coding RNA 1094

FISH fluorescence in situ hybridization

RIP RNA immunoprecipitation

ChIP chromatin immunoprecipitation

CDKN1A cyclin dependent kinase inhibitor 1A

RBMS2 RNA binding motif single stranded interacting protein 2

HDAC1 histone deacetylase 1

RUNX1 RUNX family transcription factor 1

nt nucleotides

ceRNAs competitive endogenous RNAs

EMT epithelial-to-mesenchymal transition

FBS fetal bovine serum

siRNAs small interference RNAs

qRT-PCR quantitative real-time reverse transcription polymerase chain reaction

ActD actinomycin D

CHX cycloheximide

DAPI 4,6-diamidino-2-phenylindole

TFOs triplex-forming oligonucleotides

IHC immunohistochemical

HE staining hematoxylin & eosin (HE) staining

DAB diaminobenzidine

CCK-8 cell counting kit-8

NCBI National Center for Biotechnology Information

OS overall survival

AREs AU-rich elements

3’-UTR 3’-untranslated region

H3K9 H3 lysine-9

H3K9Ac H3 lysine-9 acetylation

TrT triple target site

Ethics approval and consent to participate

Human studies were approved by the Institutional Review Board of the Nanjing Medical University, based on the Declaration of Helsinki. Written consent was obtained from each patient before surgery. Animal studies were approved by the Institutional Animal Care and Use Committee of the Laboratory Animal Center, Nanjing Medical University.

Consent for publication

Not applicable.

Availability of data and material

The authors declared that all data supporting the conclusions of this research is available.

Competing interests

No conflict of interest was declared by the authors.

Funding

The research was funded by Nanjing Medical University Science and Technology development Funding (NMUB20230152).

Authors' contributions

XY Z: Formal analysis, Visualization, Writing-Original Draft. GH C: Methodology, Formal Analysis. H L: Investigation, Writing-review & Editing. LM X: Validation, Data Curation. F Z: Data Curation. HA S: Supervision, Project administration. BJ F: Conceptualization, Supervision, Resources.

Acknowledgments

Not applicable.

H. Sung, J. Ferlay, R. L. Siegel, M. Laversanne, I. Soerjomataram, A. Jemal and F. Bray Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries CA-Cancer J. Clin. 71 (2021) 209-249. DOI: 10.3322/caac.21660.
S. C. Oh, B. H. Sohn, J. H. Cheong, S. B. Kim, J. E. Lee, K. C. Park, S. H. Lee, J. L. Park, Y. Y. Park, H. S. Lee, H. J. Jang, E. S. Park, S. C. Kim, J. Heo, I. S. Chu, Y. J. Jang, Y. J. Mok, W. Jung, B. H. Kim, A. Kim, J. Y. Cho, J. Y. Lim, Y. Hayashi, S. M. Song, E. Elimova, J. S. Estralla, J. H. Lee, M. S. Bhutani, Y. L. Lu, W. B. Liu, J. Lee, W. K. Kang, S. Kim, S. H. Noh, G. B. Mills, S. Y. Kim, J. A. Ajani and J. S. Lee Clinical and genomic landscape of gastric cancer with a mesenchymal phenotype Nat. Commun. 9 (2018) 14. DOI: 10.1038/s41467-018-04179-8.
A. M. Schmitt and H. Y. Chang Long Noncoding RNAs in Cancer Pathways Cancer Cell 29 (2016) 452-463. DOI: 10.1016/j.ccell.2016.03.010.
J. Luo, L. F. Langer and J. Liu A novel role of LncRNA in regulating tumor metabolism and angiogenesis under hypoxia Cancer Commun. 39 (2019) 3. DOI: 10.1186/s40880-019-0348-x.
M. Huarte The emerging role of IncRNAs in cancer Nat. Med. 21 (2015) 1253-1261. DOI: 10.1038/nm.3981.
A. Bhan, M. Soleimani and S. S. Mandal Long Noncoding RNA and Cancer: A New Paradigm Cancer Res. 77 (2017) 3965-3981. DOI: 10.1158/0008-5472.Can-16-2634.
S. Momtazmanesh and N. Rezaei Long Non-Coding RNAs in Diagnosis, Treatment, Prognosis, and Progression of Glioma: A State-of-the-Art Review Front. Oncol. 11 (2021) 26. DOI: 10.3389/fonc.2021.712786.
C. J. Wang, C. R. Chao, W. F. Zhao, H. M. Liu, J. S. Feng and Y. X. Cui Long noncoding RNA SNHG9 facilitates growth of glioma stem-like cells via miR-326/SOX9 axis J. Gene. Med. 24 (2022) 10. DOI: 10.1002/jgm.3334.
M. Xu, X. X. Chen, K. Lin, K. X. Zeng, X. X. Liu, B. Pan, X. N. Xu, T. Xu, X. X. Hu, L. Sun, B. S. He, Y. Q. Pan, H. L. Sun and S. K. Wang The long noncoding RNA SNHG1 regulates colorectal cancer cell growth through interactions with EZH2 and miR-154-5p Mol. Cancer 17 (2018) 16. DOI: 10.1186/s12943-018-0894-x.
P. Q. Fang, H. Chen, Z. F. Ma, C. C. Han, W. D. Yin, S. W. Wang, H. Y. Zhu, W. J. Xia, J. Wang, L. Xu, T. Y. Liu and R. Yin LncRNA LINC00525 suppresses p21 expression via mRNA decay and triplex-mediated changes in chromatin structure in lung adenocarcinoma Cancer Commun. 41 (2021) 596-614. DOI: 10.1002/cac2.12181.
R. Bai, M. Sun, Y. Chen, S. Zhuo, G. Song, T. Wang and Z. Zhang H19 recruited m6A reader YTHDF1 to promote SCARB1 translation and facilitate angiogenesis in gastric cancer Chinese medical journal (2023). DOI: 10.1097/cm9.0000000000002722.
J. L. Zheng, Q. Zhang, Z. Zhao, Y. Qiu, Y. J. Zhou, Z. P. Wu, C. Jiang, X. Wang and X. B. Jiang Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m(6)A-dependent recognition of Nrf2 mRNA mediated by SND1 J. Exp. Clin. Cancer Res. 42 (2023) 20. DOI: 10.1186/s13046-023-02684-3.
X. Wu, C. Kong and Y. L. Wu Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis Bioengineered 12 (2021) 9046-9057. DOI: 10.1080/21655979.2021.1993715.
C. Luo, K. Lin, C. G. Hu, X. J. Zhu, J. F. Zhu and Z. M. Zhu LINC01094 promotes pancreatic cancer progression by sponging miR-577 to regulate LIN28B expression and the PI3K/AKT pathway Mol. Ther.-Nucl. Acids 26 (2021) 523-535. DOI: 10.1016/j.omtn.2021.08.024.
J. Xu, P. Zhang, H. J. Sun and Y. Liu LINC01094/miR-577 axis regulates the progression of ovarian cancer J. Ovarian Res. 13 (2020) 9. DOI: 10.1186/s13048-020-00721-9.
Y. C. Ye, O. Ge, C. B. Zang, L. N. Yu, J. Eucker and Y. L. Chen LINC01094 Predicts Poor Prognosis in Patients With Gastric Cancer and is Correlated With EMT and Macrophage Infiltration Technol. Cancer Res. Treat. 21 (2022) 13. DOI: 10.1177/15330338221080977.
P. P. Xu, S. L. Liu, S. Song, X. Yao, X. C. Li, J. Zhang, Y. B. Liu, Y. Zheng, G. L. Gao and J. J. Xu Identification and validation of a novel angiogenesis-related gene signature for predicting prognosis in gastric adenocarcinoma Front. Oncol. 12 (2023) 17. DOI: 10.3389/fonc.2022.965102.
W. W. Wang, Q. S. Pei, L. F. Wang, T. Mu and H. Feng Construction of a Prognostic Signature of 10 Autophagy-Related lncRNAs in Gastric Cancer Int. J. Gen. Med. 15 (2022) 3699-3710. DOI: 10.2147/ijgm.S348943.
T. L. Yew, F. Y. Chiu, C. C. Tsai, H. L. Chen, W. P. Lee, Y. J. Chen, M. C. Chang and S. C. Hung Knockdown of p21Cip1/Waf1 enhances proliferation, the expression of stemness markers, and osteogenic potential in human mesenchymal stem cells Aging Cell 10 (2011) 349-361. DOI: 10.1111/j.1474-9726.2011.00676.x.
X. Wang, H. S. Guo, W. X. Liu, C. M. Yang, L. Yang, D. G. Wang and X. G. Wang Effects of siRNA-Mediated Knockdown of HDAC1 on the Biological Behavior of Esophageal Carcinoma Cell Lines Med. Sci. Monitor 22 (2016) 1291-1296. DOI: 10.12659/msm.895853.
X. Sun, Y. Hu, J. Wu, L. Shi, L. Zhu, P. W. Xi, J. F. Wei and Q. Ding RBMS2 inhibits the proliferation by stabilizing P21 mRNA in breast cancer J. Exp. Clin. Cancer Res. 37 (2018) 14. DOI: 10.1186/s13046-018-0968-z.
H. Y. Chen, Y. L. Liu, P. Liu, Q. X. Dai and P. L. Wang LINC01094 promotes the invasion of ovarian cancer cells and regulates the Wnt/beta-catenin signaling pathway by targeting miR-532-3p Exp. Ther. Med. 22 (2021) 12. DOI: 10.3892/etm.2021.10662.
M. S. Zhu, Y. L. Wu, Z. W. Wang, M. H. Lin, B. Su, C. Y. Li, F. L. Liang and X. J. Chen miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D Oncol. Lett. 21 (2021) 8. DOI: 10.3892/ol.2020.12413.
E. Ogawa, T. Edamitsu, H. Ohmori, K. Kohu, M. Kurokawa, H. Kiyonari, M. Satake and R. Okuyama Transcription Factors Runx1 and Runx3 Suppress Keratin Expression in Undifferentiated Keratinocytes Int. J. Mol. Sci. 23 (2022) 13. DOI: 10.3390/ijms231710039.
F. Xu, T. Xia, Q. T. Xu, X. Zhang, Y. Z. Huang, X. Sun, L. Shi, X. J. Zhou, J. F. Wei and Q. Ding RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression Int. J. Biol. Sci. 18 (2022) 1724-1736. DOI: 10.7150/ijbs.66480.
L. L. Lin, J. Xiao, L. Shi, W. W. Chen, Y. G. Ge, M. K. Jiang, Z. L. Li, H. Fan, L. Yang and Z. K. Xu STRA6 exerts oncogenic role in gastric tumorigenesis by acting as a crucial target of miR-873 J. Exp. Clin. Cancer Res. 38 (2019) 16. DOI: 10.1186/s13046-019-1450-2.
N. Leelatian, D. B. Doxie, A. R. Greenplate, J. Sinnaeve, R. A. Ihrie and J. M. Irish Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis Current protocols in molecular biology 118 (2017) 25C.21.21-25C.21.23. DOI: 10.1002/cpmb.37.
R. Kumar, A. M. Paul, R. Amjesh, B. George and M. R. Pillai Coordinated dysregulation of cancer progression by the HER family and p21-activated kinases Cancer Metastasis Rev. 39 (2020) 583-601. DOI: 10.1007/s10555-020-09922-6.
D. Kim, L. Herdeis, D. Rudolph, Y. L. Zhao, J. Böttcher, A. Vides, C. I. Ayala-Santos, Y. Pourfarjam, A. Cuevas-Navarro, J. Y. Xue, A. Mantoulidis, J. Bröker, T. Wunberg, O. Schaaf, J. Popow, B. Wolkerstorfer, K. G. Kropatsch, R. Qu, E. de Stanchina, B. Sang, C. C. Li, D. B. McConnell, N. Kraut and P. Lito Pan-KRAS inhibitor disables oncogenic signalling and tumour growth Nature 619 (2023) 160-+. DOI: 10.1038/s41586-023-06123-3.
H. Mamdani and S. I. Jalal Histone Deacetylase Inhibition in Non-small Cell Lung Cancer: Hype or Hope? Front. Cell. Dev. Biol. 8 (2020) 10. DOI: 10.3389/fcell.2020.582370.
P. Patel, S. K. Wahan, S. Vishakha, B. Das Kurmi, G. Das Gupta, H. Rajak and V. Asati Recent Progress in Histone Deacetylase (HDAC) 1 Inhibitors as Anticancer Agent Curr. Cancer Drug Targets 23 (2023) 47-70. DOI: 10.2174/1568009622666220624090542.
M. S. Leisegang, J. K. Bains, S. Seredinski, J. A. Oo, N. M. Krause, C. C. Kuo, S. Gunther, N. S. Cetin, T. Warwick, C. Cao, F. Boos, J. I. Ponce, S. Haydar, R. Bednarz, C. Valasarajan, D. C. Fuhrmann, J. Preussner, M. Looso, S. S. Pullamsetti, M. H. Schulz, H. R. A. Jonker, C. Richter, F. Rezende, R. Gilsbach, B. Pfluger-Muller, I. Wittig, I. Grummt, T. Ribarska, I. G. Costa, H. Schwalbe and R. P. Brandes HIF1 alpha-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex Nat. Commun. 13 (2022) 20. DOI: 10.1038/s41467-022-34252-2.
H. Pan, H. X. Wang, X. Y. Zhang, F. Yang, X. Q. Fan and H. Zhang Chromosomal instability-associated MAT1 lncRNA insulates MLL1-guided histone methylation and accelerates tumorigenesis Cell Reports 41 (2022) 25. DOI: 10.1016/j.celrep.2022.111829.
C. Zhang, D. P. Wang, Y. J. Hao, S. H. Wu, J. J. Luo, Y. C. Xue, D. Wang, G. H. Li, L. H. Liu, C. W. Shao, H. Y. Li, J. F. Yuan, M. X. Zhu, X. D. Fu, X. Yang, R. S. Chen and Y. Teng LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly Mol. Cell 82 (2022) 4018-+. DOI: 10.1016/j.molcel.2022.09.022.
M. Rivas, M. E. Johnston, R. Gulati, M. Kumbaji, T. F. M. Aguiar, L. Timchenko, A. Krepischi, S. Shin, A. Bondoc, G. Tiao, J. Geller and N. Timchenko HDAC1-Dependent Repression of Markers of Hepatocytes and P21 Is Involved in Development of Pediatric Liver Cancer Cell. Mol. Gastroenterol. Hepatol. 12 (2021) 1669-1682. DOI: 10.1016/j.jcmgh.2021.06.026.
H. Y. Zhou, Y. Cai, D. N. Liu, M. H. Li, Y. Sha, W. L. Zhang, K. Wang, J. P. Gong, N. Tang, A. L. Huang and J. Xia Pharmacological or transcriptional inhibition of both HDAC1 and 2 leads to cell cycle blockage and apoptosis via p21(Waf1/Cip1) and p19(INK4d) upregulation in hepatocellular carcinoma Cell Prolif. 51 (2018) 14. DOI: 10.1111/cpr.12447.
M. LeBoeuf, A. Terrell, S. Trivedi, S. Sinha, J. A. Epstein, E. N. Olson, E. E. Morrisey and S. E. Millar Hdac1 and Hdac2 Act Redundantly to Control p63 and p53 Functions in Epidermal Progenitor Cells Dev. Cell 19 (2010) 807-818. DOI: 10.1016/j.devcel.2010.10.015.
A. F. Thomas, G. L. Kelly and A. Strasser Of the many cellular responses activated by TP53, which ones are critical for tumour suppression? Cell Death Differ. 29 (2022) 961-971. DOI: 10.1038/s41418-022-00996-z.
W. K. K. Wu, C. H. Cho, C. W. Lee, D. M. Fan, K. C. Wu, J. Yu and J. J. Y. Sung Dysregulation of cellular signaling in gastric cancer Cancer Lett. 295 (2010) 144-153. DOI: 10.1016/j.canlet.2010.04.025.
X. M. Zeng, J. Xiao, X. Bai, Y. W. Liu, M. L. Zhang, J. R. Liu, Z. X. Lin and Z. W. Zhang Research progress on the circRNA/lncRNA-miRNA-mRNA axis in gastric cancer Pathol. Res. Pract. 238 (2022) 15. DOI: 10.1016/j.prp.2022.154030.
B. C. Ma, S. H. Wang, W. Z. Wu, P. F. Shan, Y. F. Chen, J. Q. Meng, L. P. Xing, J. Y. Yun, L. H. Hao, X. Y. Wang, S. Y. Li and Y. H. Guo Mechanisms of circRNA/lncRNA-miRNA interactions and applications in disease and drug research Biomed. Pharmacother. 162 (2023) 12. DOI: 10.1016/j.biopha.2023.114672.
J. Han, C. A. LaVigne, B. T. Jones, H. Zhang, F. Gillett and J. T. Mendell A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming Science 370 (2020) 1432-+. DOI: 10.1126/science.abc9546.
D. Pelletier, B. Rivera, M. R. Fabian and W. D. Foulkes miRNA biogenesis and inherited disorders: clinico-molecular insights Trends in genetics : TIG 39 (2023) 401-414. DOI: 10.1016/j.tig.2023.01.009.
R. Sood, Y. Kamikubo and P. Liu Role of RUNX1 in hematological malignancies Blood 129 (2017) 2070-2082. DOI: 10.1182/blood-2016-10-687830.
T. C. Lin RUNX1 and cancer Biochim. Biophys. Acta-Rev. Cancer 1877 (2022) 12. DOI: 10.1016/j.bbcan.2022.188715.
Y. Zhang, X. Wang, C. Zhang and H. Yi lncRNAs: the abyss being gazed back Drug discovery today (2023) 103664. DOI: 10.1016/j.drudis.2023.103664.
L. D. Meng, Y. H. Zhang, P. F. Wu, D. R. Li, Y. C. Lu, P. Shen, T. Y. Yang, G. D. Shi, Q. Chen, H. Yuan, W. L. Ge, Y. Miao, M. Tu and K. R. Jiang CircSTX6 promotes pancreatic ductal adenocarcinoma progression by sponging miR-449b-5p and interacting with CUL2 Mol. Cancer 21 (2022) 23. DOI: 10.1186/s12943-022-01599-5.
S. K. Qiu, B. W. Li, Y. W. Xia, Z. Xuan, Z. Li, L. Xie, C. Gu, J. L. Lv, C. Lu, T. L. Jiang, L. Fang, P. H. Xu, J. Yang, Y. Li, Z. T. Chen, L. Zhang, L. J. Wang, D. A. C. Zhang, H. Xu, W. Z. Wang and Z. K. Xu CircTHBS1 drives gastric cancer progression by increasing INHBA mRNA expression and stability in a ceRNA- and RBP-dependent manner Cell Death Dis. 13 (2022) 15. DOI: 10.1038/s41419-022-04720-0.
Q. Wang, C. Chen, X. Xu, C. J. Shu, C. C. Cao, Z. D. Wang, Y. Fu, L. Xu, K. Y. Xu, J. W. Xu, A. L. Xia, B. Wang, G. F. Xu, X. P. Zou, R. B. Su, W. Kang, Y. C. Xue, R. Mo, B. C. Sun and S. Y. Wang APAF1-Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly Adv. Sci. 9 (2022) 18. DOI: 10.1002/advs.202201889.
Y. L. Wang, J. Y. Liu, J. E. Yang, X. M. Yu, Z. L. Chen, Y. J. Chen, M. Kuang, Y. Zhu and S. M. Zhuang Lnc-UCID Promotes G1/S Transition and Hepatoma Growth by Preventing DHX9-Mediated CDK6 Down-regulation Hepatology 70 (2019) 259-275. DOI: 10.1002/hep.30613.
F. A. Buske, D. C. Bauer, J. S. Mattick and T. L. Bailey Triplexator: Detecting nucleic acid triple helices in genomic and transcriptomic data Genome Res. 22 (2012) 1372-1381. DOI: 10.1101/gr.130237.111.
C. H. Chen, W. He, J. Huang, B. Wang, H. Li, Q. Q. Cai, F. Su, J. M. Bi, H. W. Liu, B. Zhang, N. Jiang, G. Z. Zhong, Y. Zhao, W. Dong and T. X. Lin LNMAT1 promotes lymphatic metastasis of bladder cancer via CCL2 dependent macrophage recruitment Nat. Commun. 9 (2018) 18. DOI: 10.1038/s41467-018-06152-x.
T. Mondal, S. Subhash, R. Vaid, S. Enroth, S. Uday, B. Reinius, S. Mitra, A. Mohammed, A. R. James, E. Hoberg, A. Moustakas, U. Gyllensten, S. J. M. Jones, C. M. Gustafsson, A. H. Sims, F. Westerlund, E. Gorab and C. Kanduri MEG3 long noncoding RNA regulates the TGF-beta pathway genes through formation of RNA-DNA triplex structures Nat. Commun. 6 (2015) 17. DOI: 10.1038/ncomms8743.
E. Y. Hattori, T. Masuda, Y. Mineharu, M. Mikami, Y. Terada, Y. Matsui, H. Kubota, H. Matsuo, M. Hirata, T. R. Kataoka, T. Nakahata, S. Ikeda, S. Miyamoto, H. Sugiyama, Y. Arakawa and Y. Kamikubo A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice (vol 5, 939, 2022) Commun. Biol. 5 (2022) 1. DOI: 10.1038/s42003-022-04006-3.
Y. J. Na, A. Hall, Y. A. Yu, L. Hu, K. Choi, J. A. Burgard, S. Szabo, G. Huang, N. Ratner and J. Q. Wu Runx1/3-driven adaptive endoplasmic reticulum stress pathways contribute to neurofibromagenesis Oncogene 42 (2023) 1038-1047. DOI: 10.1038/s41388-023-02620-x.
L. S. Zhang, F. B. Fried, H. Guo and A. D. Friedman Cyclin-dependent kinase phosphorylation of RUNX1/AML1 on 3 sites increases transactivation potency and stimulates cell proliferation Blood 111 (2008) 1193-1200. DOI: 10.1182/blood-2007-08-109702.

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RUNX1-mediated LINC01094 promotes gastric cancer through dual targeting of CDKN1A by directly binding RBMS2 and HDAC1

Status:

Journal Publication

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Abstract

Background

Methods

Results

Conclusions

Figures

Introduction

Methods

Tissue samples

Cell culture

Plasmid construction, siRNA transfection, and lentiviral infection

RNA extraction and qRT-PCR

Nuclear-cytoplasmic fractionation

mRNA stability and protein stability analyzes

Fluorescence in situ hybridization (FISH) assay

RNA pull-down assay

RNA immunoprecipitation (RIP) assay

Chromatin immunoprecipitation (ChIP) assay

Triplex capture assay

Luciferase reporter assay

Western blot analysis

Immunohistochemical (IHC) and hematoxylin & eosin (HE) staining

Functional assays

Cell proliferation, migration, and invasion assays

Flow cytometric analysis

Animal experiments

GC organoid model

Bioinformatic and statistical analyzes

Results

Identification and characteristics of LINC01094 in GC

LINC01094 promotes malignant behaviors of GC cells in vitro and in vivo

LINC01094 decreases CDKN1A transcription by recruiting HDAC1 to the CDKN1A promoter

LINC01094 exerts biological effects by downregulating CDKN1A

LINC01094 upregulates RUNX1 by sponging miR-128-3p

LINC01094 is upregulated by RUNX1

Discussion

Conclusion

Abbreviations

Declarations

References

Additional Declarations

Supplementary Files

Status:

Journal Publication

Version 1